M Kitamura Y Takayama S Kojima K Kohno H Ogata Y Higuchi H Inoue
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1676(2) 172-181 2004年1月
The gene encoding an enolase from Desulfovibrio vulgaris (Miyazaki F) was cloned and overexpressed in Escherichia coli. A 2.1-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by double digestion with PstI and BamHI, contained an enolase gen...
M Kitamura Y Takayama S Kojima K Kohno H Ogata Y Higuchi H Inoue
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1676(2) 172-181 2004年1月
The gene encoding an enolase from Desulfovibrio vulgaris (Miyazaki F) was cloned and overexpressed in Escherichia coli. A 2.1-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by double digestion with PstI and BamHI, contained an enolase gen...
H Hemmi T Kumazaki T Yamazaki S Kojima T Yoshida Y Kyogoku M Katsu F Shinohara H Yokosawa K Miura Y Kobayashi
BIOCHEMISTRY 42(9) 2524-2534 2003年3月
The ovomucoid third domain from silver pheasant (OMSVP3), a typical Kazal-type inhibitor, strongly inhibits different serine proteases of various specificities, i.e., chymotrypsin, Streptomyces griseus protease, subtilisin, and elastase. Structura...
H Hemmi T Kumazaki T Yamazaki S Kojima T Yoshida Y Kyogoku M Katsu F Shinohara H Yokosawa K Miura Y Kobayashi
BIOCHEMISTRY 42(9) 2524-2534 2003年3月
The ovomucoid third domain from silver pheasant (OMSVP3), a typical Kazal-type inhibitor, strongly inhibits different serine proteases of various specificities, i.e., chymotrypsin, Streptomyces griseus protease, subtilisin, and elastase. Structura...
M Oda A Tamura K Kanaori S Kojima K Miura K Momma B Tonomura K Akasaka
JOURNAL OF BIOCHEMISTRY 132(6) 991-995 2002年12月
Single amino acid mutations of Met103 in the hydrophobic core of a serine protease inhibitor, Streptomyces subtilisin inhibitor, caused little change in the inhibitory activity, as measured by the inhibitor constant, although some altered the ther...
人文科学研究科
理学部