Curriculum Vitaes

Takayuki Nishizaka

(西坂崇之)

Profile Information

Affiliation: Faculty of Science, Department of Physics, Gakushuin University

Degree: 博士（理学）

J-GLOBAL ID: 200901031435622136
researchmap Member ID: 5000060767

External link: http://www.gakushuin.ac.jp/univ/sci/phys/nishizaka/lab/lp/

Research Areas

1

Life sciences / Biophysics /

Research History

8

Education

2

- 1996

Waseda University
- 1991

School of Science and Engineering, Waseda University

Awards

1

Mar, 2009

Japan Society for the Promotion of Science, Single-Molecule Studies of Structure-Function Coupling in Protein Machines

NISHIZAKA Takayuki

Papers

151

Conformational Changes in the beta Subunits of F-1-Atpase Revealed by Fret Measurements During the Rotation of the gamma Subunit

Mitsuhiro Sugawa, Masaru Kobayashi, Takashi Matsui, Tomoko Masaike, Takayuki Nishizaka

BIOPHYSICAL JOURNAL, 106(2) 252A-252A, Jan, 2014 Peer-reviewed
1P160 Bacterium moves like a tank(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Nakane Daisuke, Sato Keiko, Wada Hirofumi, McBride Mark, Nakayama Koji, Nishizaka Takayuki

Seibutsu Butsuri, 53(1) S132, 2013
3P175 Three-dimensional motion of an isolated murine tracheal cilium under load(12. Cell biology,Poster)

Kato Takanobu, Iwase Toshihito, Masaike Tomoko, Ikegami Koji, Setou Mitsutoshi, Nishizaka Takayuki

Seibutsu Butsuri, 53(1) S240, 2013
1P163 Measurement of lifetime of the bond between the shaft and the cylinder in single F_1-ATPase(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Naito Tatsuya, Okada Kaoru, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 53(1) S132, 2013
1P158 Nucleotide-dependent interaction between legs of Mycoplasma mobile and sialyllactose(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Kinosita Yoshiaki, Nakane Daisuke, Miyata Makoto, Nishizaka Takayuki

Seibutsu Butsuri, 53(1) S132, 2013
1P175 The relationship between F_1-ATPase P-loop mutants and Pi release(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Yoshida Hikaru, Ito Ayumi, Ito Jotaro, Masaike Tomoko, Nishizaka Takayuki, Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

Seibutsu Butsuri, 53(1) S134, 2013
1P161 Advanced TIRF microscopy to detect single-molecule conformational changes in both azimuth and axial axis using polarization switching(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Mikami Nagisa, Masaike Tomoko, Sugawa Mitsuhiro, Nishizaka Takayuki

Seibutsu Butsuri, 53(1) S132, 2013
Super-resolution imaging of microtubular transport in vitro based on nanoscale localization sampling using nanoantenna arrays.

Kim K, Yajima J, Oh Y, LeeW, Oowada S, Nishizaka T, Kim D

Small, 786 892-900, 2013 Peer-reviewed
Controlled rotation of the F-1-ATPase reveals differential and continuous binding changes for ATP synthesis

Kengo Adachi, Kazuhiro Oiwa, Masasuke Yoshida, Takayuki Nishizaka, Kazuhiko Kinosita

NATURE COMMUNICATIONS, 3 1022, Aug, 2012 Peer-reviewed
Direct Observation of Strand Passage by DNA-Topoisomerase and Its Limited Processivity

Katsunori Yogo, Taisaku Ogawa, Masahito Hayashi, Yoshie Harada, Takayuki Nishizaka, Kazuhiko Kinosita

PLOS ONE, 7(4) e34920, Apr, 2012 Peer-reviewed
Imaging: Nanoscale Localization Sampling Based on Nanoantenna Arrays for Super‐resolution Imaging of Fluorescent Monomers on Sliding Microtubules (Small 6/2012)

Kyujung Kim, Junichiro Yajima, Youngjin Oh, Wonju Lee, Shinsuke Oowada, Takayuki Nishizaka, Donghyun Kim

Small, 8(6) 786-786, Mar 19, 2012
Nanoscale Localization Sampling Based on Nanoantenna Arrays for Super-resolution Imaging of Fluorescent Monomers on Sliding Microtubules

Kyujung Kim, Junichiro Yajima, Youngjin Oh, Wonju Lee, Shinsuke Oowada, Takayuki Nishizaka, Donghyun Kim

SMALL, 8(6) 892-900, Mar, 2012 Peer-reviewed
3E1022 Direct electron microscopy of protein crystals and Mvconlasma cells in solution using the Atmosnheric SEM(Proteins:Structure,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Maruyama Yuusuke, Ebihara Tatsuhiko, Nakane Daisuke, Nishiyama Hidetoshi, Nishizaka Takayuki, Senda Miki, Mio Kazuhiro, Suga Mitsuo, Senda Toshiya, Miyata Makoto, Sato Chikara

Seibutsu Butsuri, 52 S64, 2012
2PS023 Directed binding of Mycoplasma mobile may cause directed displacement in gliding(The 50th Annual Meeting of the Biophysical Society of Japan)

Tanaka Akihiro, Nakane Daisuke, Nishizaka Takayuki, Miyata Makoto

Seibutsu Butsuri, 52 S113-S114, 2012
1PS025 Conformational changes in the β subunits of F_1-ATPase revealed by FRET measurements during the rotation of the γ subunit(The 50th Annual Meeting of the Biophysical Society of Japan)

Sugawa Mitsuhiro, Kobayashi Masaru, Matsui Takashi, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 52 S78, 2012
3PS030 Combining of isotropic TIRFM and polarization-modulation TIRFM(The 50th Annual Meeting of the Biophysical Society of Japan)

Fujimura Shoko, Hasimoto Yuh, Adachi Kengo, Nakayama Rinako, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 52 S151, 2012
3A1034 UNBINDING FORCE MEASUREMENTS OF THE SHAFT FROM THE CYLINDER OF F1-ATPase(Molecular Motors III:F1 ATPase and Mycoplasma,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Naito Tatsuya, Okada Kaoru, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 52 S57, 2012
3A0912 Motions in F_1-ATPase and ciliary axonemes that drive functions(Molecular Motors III:F1 ATPase and Mycoplasma,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Masaike Tomoko, Ikegami Koji, Nakayama Rinako, Setou Mitsutoshi, Nishizaka Takayuki

Seibutsu Butsuri, 52 S56, 2012
3A1058 Detection of steps of Mycoplasma mobile gliding ghost(Molecular Motors III:F1 ATPase and Mycoplasma,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Kinosita Yoshiaki, Nakane Daisuke, Mizutani Kana, Miyata Makoto, Nishizaka Takayuki

Seibutsu Butsuri, 52 S57, 2012
2H1448 Three-dimensional tracking of gliding actin filament in an in vitro motility assay(Muscle,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Naganawa Tatsuya, Shimozawa Togo, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 52 S52, 2012
Rotational catalysis by F1-ATPase

K. Adachi, T. Nishizaka, K. Kinosita

Comprehensive Biophysics, 8 35-49, 2012 Peer-reviewed
Rapid imaging of mycoplasma in solution using Atmospheric Scanning Electron Microscopy (ASEM).

Sato C, Manaka S, Nakane D, Nishiyama H, Suga M, Nishizaka T, Miyata M, Maruyama Y

Biochemical and biophysical research communications, 417(4) 1213-1218, Jan, 2012 Peer-reviewed
A Change in the Radius of Rotation of F-1-ATPase Indicates a Tilting Motion of the Central Shaft

Mitsuhiro Sugawa, Kaoru A. Okada, Tomoko Masaike, Takayuki Nishizaka

BIOPHYSICAL JOURNAL, 101(9) 2201-2206, Nov, 2011 Peer-reviewed
Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells

Toshikazu Tsuji, Shigeko Kawai-Noma, Chan-Gi Pack, Hideki Terajima, Junichiro Yajima, Takayuki Nishizaka, Masataka Kinjo, Hideki Taguchi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 405(4) 638-643, Feb, 2011 Peer-reviewed
1L1324 Development of circular orientation fluorescence emitter imaging (COFEI) with the advanced TIRF microscopy(Molecular motor 1,The 49th Annual Meeting of the Biophysical Society of Japan)

Sugawa Mitsuhiro, Fujii Fumihiko, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 51 S60, 2011
1L1448 Observation of rotation of mutant F1-ATPase with twisted A at high temporal resolution(Molecular motor 1,The 49th Annual Meeting of the Biophysical Society of Japan)

Hasimoto Yuh, Sugawa Mitsuhiro, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 51 S61, 2011
1L1412 P44 Manipulating the Nucleotides Affinity in Rotary Motor of F_1-ATPase by Forced Rotation(Molecular motor 1,The 49th Annual Meeting of the Biophysical Society of Japan)

Adachi Kengo, Oiwa Kazuhiro, Nishizaka Takayuki, Yoshida Masasuke, Kinosita Jr. Kazuhiko

Seibutsu Butsuri, 51 S60-S61, 2011
Simultaneous observation of chemomechanical coupling of a molecular motor

Takayuki Nishizaka, Yuh Hasimoto, Tomoko Masaike

Methods in Molecular Biology, 778 259-271, 2011 Peer-reviewed
1P175 1B1255 Application of 3-D prismatic optical tracking : single-molecule optical tweezers approach and stereoscopic 3-D view(Molecular motor,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)

Nishizaka Takayuki, Adachi Kengo, Tatsuguchi Ayako, Masaike Tomoko

Seibutsu Butsuri, 50(2) S50, 2010
2P179 Corkscrew motion of a sliding microtubule driven by single-headed kinesins with mutations in N-terminal region(The 48th Annual Meeting of the Biophysical Society of Japan)

Owada Sinsuke, Yajima Junichiro, Masaike Tomoko, Tatsuguchi Ayako, Nishizaka Takayuki

Seibutsu Butsuri, 50(2) S114, 2010
2P201 Tilting of the γ subunit and conformational changes of the α_3β_3 in F1-ATPase(The 48th Annual Meeting of the Biophysical Society of Japan)

Sugawa Mitsuhiro, Okada Kaoru, Kobayashi Masaru, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 50(2) S118, 2010
2P180 High-speed imaging of single fluorescently labeled nucleotides bound to F_1-ATPase(The 48th Annual Meeting of the Biophysical Society of Japan)

Hashimoto Yu, Adachi Kengo, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 50(2) S114, 2010
Molecular Bio-Motors in Living Cells

Takayuki Nishizaka

NANO/MICRO BIOTECHNOLOGY, 119 3-15, 2010 Peer-reviewed
ポスドクは超（チョー）楽しい！

西坂崇之

生物物理, 50(4) 155, 2010 Peer-reviewed
科学の“雲の通ひ路”

西坂崇之

生物物理, 50(5) 214-215, 2010 Peer-reviewed
Watching single molecule under optical microscopes

Microscopy, 45(3) 173-179, 2010 Peer-reviewed
[Visualisation of conformational changes of proteins at the single-molecule level].

Masaike T, Nishizaka T

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 54(15) 1919-1927, Dec, 2009 Peer-reviewed
Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane

Kazuhito V. Tabata, Ken Sato, Toru Ide, Takayuki Nishizaka, Akihiko Nakano, Hiroyuki Noji

EMBO JOURNAL, 28(21) 3279-3289, Nov, 2009 Peer-reviewed
1SP6-01 Visualization of COPII vesicle formation process reconstituted in the artifical lipid bilayer.(1SP6 Membrane transformers!! : The combine and the dissociation to change the shape of biomembrane,The 47th Annual Meeting of the Biophysical Society of Japan)

Tabata Kazuhito, Sato Ken, Ide Toru, Nishizaka Takayuki, Nakano Akihiko, Noji Hiroyuki

Seibutsu Butsuri, 49 S7-S8, 2009
1P-125 Conformational changes of the β subunits in F_1-ATPase revealed by single molecule FRET measurement(Molecular motor, The 47th Annual Meeting of the Biophysical Society of Japan)

Sugawa Mitsuhiro, Kobayashi Masaru, Nishizaka Takayuki, Masaike Tomoko

Seibutsu Butsuri, 49 S83, 2009
1P-123 High-Accuracy Localization of Single Nucleotides Bound to the Catalytic Sites in Rotary Motor F_1-ATPase(Molecular motor, The 47th Annual Meeting of the Biophysical Society of Japan)

Adachi Kengo, Masaike Tomoko, Oiwa Kazuhiro, Nishizaka Takayuki

Seibutsu Butsuri, 49 S82, 2009
1P-124 Versatility of the unbinding force measurements at the single-molecule level adapted to different molecular motors(Molecular motor, The 47th Annual Meeting of the Biophysical Society of Japan)

Mikhailenko Sergey, Oguchi Yusuke, Ohki Takashi, Olivares Adrian O., Cruz Enrique M. De La, Ishiwata Shin'ichi, Masaike Tomoko, Nishizaka Takayuki

Seibutsu Butsuri, 49 S83, 2009
1TA4-03 Step detection of gliding bacterium, Mycoplasma mobile(The 47th Annual Meeting of the Biophysical Society of Japan)

Nakane Daisuke, Nishizaka Takayuki, Miyata Makoto

Seibutsu Butsuri, 49 S29, 2009
蛋白質の局所構造変化を1個のレベルで可視化する

政池知子, 西坂崇之

蛋白質・核酸・酵素, 54 1919-1927, 2009 Peer-reviewed
Cooperative three-step motions in catalytic subunits of F(1)-ATPase correlate with 80 degrees and 40 degrees substep rotations

Tomoko Masaike, Fumie Koyama-Horibe, Kazuhiro Oiwa, Masasuke Yoshida, Takayuki Nishizaka

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 15(12) 1326-1333, Dec, 2008 Peer-reviewed
Cooperative three-step motions in catalytic subunits of F 1-ATPase correlate with 80° and 40° substep rotations

Tomoko Masaike, Fumie Koyama-Horibe, Kazuhiro Oiwa, Masasuke Yoshida, Takayuki Nishizaka

Nature Structural and Molecular Biology, 15(12) 1326-1333, Dec, 2008 Peer-reviewed
A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking

Junichiro Yajima, Kana Mizutani, Takayuki Nishizaka

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 15(10) 1119-1121, Oct, 2008 Peer-reviewed
A torque component present in mitotic kinesin Eg5 revealed by three-dimensional tracking

Junichiro Yajima, Kana Mizutani, Takayuki Nishizaka

Nature Structural and Molecular Biology, 15(10) 1119-1121, Oct, 2008 Peer-reviewed
2P-163 Detection of conformational changes of the β subunits in F_1-ATPase by FRET(The 46th Annual Meeting of the Biophysical Society of Japan)

KOBAYASHI Masaru, NISHIZAKA Takayuki, MASAIKE Tomoko

Seibutsu Butsuri, 48 S100, 2008
2P-171 Angle Dependence of the Affinity for Nucleotides in Rotary Motor F_1-ATPase(The 46th Annual Meeting of the Biophysical Society of Japan)

Adachi Kengo, Oiwa Kazuhiro, Nishizaka Takayuki, Noji Hiroyuki, Itoh Hiroyasu, Yoshida Masasuke, Kinosita Kazuhiko

Seibutsu Butsuri, 48 S101, 2008

Misc.

72

生態,生理・構造,遺伝・ゲノミクス・バイオテクノロジー Mycoplasma pneumoniaeの滑走運動性の分子速度計測法(Molecular speedometer in the gliding motility of Mycoplasma pneumoniae)

村田幸樹, 中根大介, 見理剛, 柴山恵吾, 西坂崇之

日本細菌学雑誌, 74(1) 37-37, Mar, 2019
シアノバクテリアの運動

中根大介, 西坂崇之

生物工学会誌, 96(5) 244‐247, May, 2018 Peer-reviewedInvited
光ピンセットによるマニピュレーション技術とその繊毛への応用

加藤孝信, 西坂崇之

実験医学, 36(6) 974‐975, Apr, 2018 Invited
普遍的な細胞小器官である“繊毛”の形状変化の定量的解析へ向けたツールの作成

西坂崇之, 横山悦郎, 加藤孝信

学習院大学計算機センター年報, 38 72‐76, Mar, 2018
マイコプラズマの滑走速度を示す分子速度計(Molecular speedometer indicating gliding speed in Mycoplasma pneumoniae)

村田幸樹, 見理剛, 中根大介, 柴山恵吾, 西坂崇之

日本細菌学雑誌, 73(1) 82-82, Feb, 2018

Presentations

44

クラミドモナス除膜モデルは負の重力走性を示すか？

鹿毛あずさ, 一村涼太, 西坂崇之

生体運動研究合同班・2025年班会議, Jan 7, 2025
赤潮形成ラフィド藻シャットネラにおける遊泳速さと前鞭毛長の相関

藤田悠作, 鹿毛あずさ, 西坂崇之

生体運動研究合同班・2025年班会議, Jan 7, 2025
Insights into the mechanism of ATP-driven rotary motors from direct torque measurement

Takayuki Nishizaka

International Centre for Interdisciplinary Science Education (ICISE), Sep 17, 2023 Invited
Insights into the Mechanism of ATP-Driven Rotary Motors from Direct Torque Measurement

Takayuki Nishizaka

11th International Conference on Biological Physics, Aug 18, 2023
光ピンセットで明らかになったマイコプラズマモービレ滑走運動の化学力学共役

木下佳昭, 中根大介, 宮田真人, 西坂崇之

生体運動研究合同班・2014年班会議, Jan 10, 2014

Professional Memberships

2

Research Projects

18

Exploring new rotary molecular motor in living organism

Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2022 - Mar, 2026
Exploring new rotary molecular motor in living organism

Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2022 - Mar, 2026
T4P dynamics in response to mechanosensing system

Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

Nakane Daisuke, Nishizaka Takayuki
Exploring of a new molecular motor in archaeal flagella

Grants-in-Aid for Scientific Research, Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2019

Nishizaka Takayuki
バクテリア巨大渦状運動の極性形成メカニズム

科学研究費助成事業, 日本学術振興会, Apr, 2016 - Mar, 2018

西坂崇之

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