Genetic and molecular biological studies on the silkworm and other lepidopteran insects. Special interests in development, reproduction, physiology, behavior, and evolution.
Following baculovirus infection, the level of host messenger RNA (mRNA) declines and the synthesis of host proteins is shutoff in virus-infected cells. To comprehensively understand the regulation of host gene expression by Bombyx mori nucleopolyhedrovirus (BmNPV), we took a complementary DNA (cDNA) subtraction approach. Northern blot analysis was then performed to confirm whether obtained clones are differentially expressed. From these analyses, we obtained 4 down-, 7 up- and 2 non-regulated host gene candidates during the early stage of infection. Sequence analyses showed that these may encode proteins involved in transcription, cell cycle, cell adhesion, protein degradation, apoptosis or energy metabolism. We then measured the ADP/ATP ratio to address the response of host energy metabolism upon baculovirus infection. The ADP/ATP ratio showed cell death-like rapid increase following BmNPV infection with a transient decrease during 12-24 h post-infection (p.i.). Taken together, our results suggest that general events in host cells are controlled at the level of mRNA expression by BmNPV infection.
Miao XX, Xub SJ, Li MH, Li MW, Huang JH, Dai FY, Marino SW, Mills DR, Zeng P, Mita K, Jia SH, Zhang Y, Liu WB, Xiang H, Guo QH, Xu AY, Kong XY, Lin HX, Shi YZ, Lu G, Zhang X, Huang W, Yasukochi Y, Sugasaki T, Shimada T, Nagaraju J, Xiang ZH, Wang SY, Goldsmith MR, Lu C, Zhao GP, Huang YP
Proceedings of the National Academy of Sciences of the United States of America, 102(45) 16303-16308, Nov 8, 2005 Peer-reviewed
Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin-I T, Abe H, Shimada T, Morishita S, Sasaki T
DNA research : an international journal for rapid publication of reports on genes and genomes, 11(1) 27-35, Feb 2, 2004 Peer-reviewed
Mita, K., Morimyo, M., Okano, K., Koike, Y., Nohata, J., Kawasaki, H., Kadono-Okuda, K., Yamamoto, K., Suzuki, M.G., Shimada, T., Goldsmith, M.R., Maeda, S.
Proceedings of the National Academy of Sciences of the United States of America, 100(SUPPL. 2) 14121-14126, 2003 Peer-reviewed
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jul, 2021 - Mar, 2025