小島修一

コジマシユウイチ (Shuichi Kojima)

基本情報

所属: 学習院大学理学部生命科学科理学部生命科学科教授

学位: 理学士(千葉大学)
理学修士(千葉大学)
工学博士(東京大学)

J-GLOBAL ID: 200901087624745392
researchmap会員ID: 1000068615

研究キーワード

研究分野

経歴

1991年 - 2007年

:学習院大学理学部助教授
1991年 - 2007年

:Associate Professor, Faculty of Science, Gakushuin University
2007年

-:学習院大学理学部教授
2007年

-:Professor, Faculty of Science, Gakushuin University
1989年 - 1991年

:東京大学工学部助手

もっとみる

学歴

もっとみる

MISC

100

New subtilisin-trypsin inhibitors produced by Streptomyces: Primary structures and their relationship to other proteinase inhibitors from Streptomyces

M Terabe, S Kojima, S Taguchi, H Momose, K Miura

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1292(2) 233-240 1996年2月
Taxonomic characterization of closely related Streptomyces spp Based on the amino acid sequence analysis of protease inhibitor proteins

S Taguchi, S Kojima, K Miura, H Momose

FEMS MICROBIOLOGY LETTERS 135(2-3) 169-173 1996年1月
Dynamics of the three methionyl side chains of Streptomyces subtilisin inhibitor. Deuterium NMR studies in solution and in the solid state

A Tamura, M Matsushita, A Naito, S Kojima, KI Miura, K Akasaka

PROTEIN SCIENCE 5(1) 127-139 1996年1月
STREPTOMYCES SERINE-PROTEASE (SAM-P20) - RECOMBINANT PRODUCTION, CHARACTERIZATION, AND INTERACTION WITH ENDOGENOUS PROTEASE INHIBITOR

S TAGUCHI, M SUZUKI, S KOJIMA, K MIURA, H MOMOSE

JOURNAL OF BACTERIOLOGY 177(22) 6638-6643 1995年11月
THE AMINO-ACID-SEQUENCE AND INTERACTION WITH THE NUCLEOSOME CORE DNA OF TRANSITION PROTEIN 4 FROM BOAR LATE SPERMATID NUCLEI

K AKAMA, H ICHIMURA, H SATO, S KOJIMA, KI MIURA, H HAYASHI, Y KOMATSU, M NAKANO

EUROPEAN JOURNAL OF BIOCHEMISTRY 233(1) 179-185 1995年10月
A SUBTILISIN INHIBITOR PRODUCED BY STREPTOMYCES-BIKINIENSIS POSSESSES A GLUTAMINE RESIDUE AT REACTIVE-SITE P1

M TERABE, S KOJIMA, S TAGUCHI, H MOMOSE, K MIURA

JOURNAL OF BIOCHEMISTRY 117(3) 609-613 1995年3月
A thermodynamic study of mutant forms of Streptomyces subtilisin inhibitor. II. Replacements at the interface of dimer formation, Val13

小島修一, Atsuo Tamura, Shuichi Kojima, Kin-ichro Miura, Julian M. Sturtevant

Journal of Molecular Biology 249(3) 636-645 1995年
A subtilisin inhibitor produced by Streptomyces bikiniensis possesses a glutamine residue at reactive site P1

KOJIMA SHUICHI, Mahito Terabe, Shuichi Kojima, Seiichi Taguchi, Haruo Momose, Kin-ichiro Miura

The Journal of Biochemistry 117(3) 609-613 1995年
A thermodynamic study of mutant forms of Streptomyces subtilisin inhibitor. II. Replacements at the interface of dimer formation, Val13

KOJIMA SHUICHI, Atsuo Tamura, Shuichi Kojima, Kin-ichro Miura, Julian M. Sturtevant

Journal of Molecular Biology 249(3) 636-645 1995年
Streptomyces serine protease (SAM-P20): Recombinant production,characterization, and interaction with endogenous protease inhibitor

KOJIMA SHUICHI, Seiichi Taguchi, Masayuki Suzuki, Shuichi Kojima, Kin-ichiro Miura, Haruo Momose

Journal of Bacteriology 177(22) 6638-6643 1995年
PRIMARY STRUCTURE AND INHIBITORY PROPERTIES OF A SUBTILISIN-CHYMOTRYPSIN INHIBITOR FROM STREPTOMYCES-VIRGINIAE

M TERABE, S KOJIMA, S TAGUCHI, H MOMOSE, K MIURA

EUROPEAN JOURNAL OF BIOCHEMISTRY 226(2) 627-632 1994年12月
EFFECT OF AN INTERSUBUNIT DISULFIDE BOND ON THE STABILITY OF STREPTOMYCES SUBTILISIN INHIBITOR

A TAMURA, S KOJIMA, K MIURA, JM STURTEVANT

BIOCHEMISTRY 33(48) 14512-14520 1994年12月
RENATURATION OF THE MATURE SUBTILISIN BPN' IMMOBILIZED ON AGAROSE BEADS

T HAYASHI, M MATSUBARA, D NOHARA, S KOJIMA, K MIURA, T SAKAI

FEBS LETTERS 350(1) 109-112 1994年8月
PRIMARY STRUCTURE AND INHIBITORY PROPERTIES OF A PROTEINASE-INHIBITOR PRODUCED BY STREPTOMYCES-CACAOI

S KOJIMA, M TERABE, S TAGUCHI, H MOMOSE, KI MIURA

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1207(1) 120-125 1994年7月
SECRETORY PRODUCTION OF CHICKEN OVOMUCOID DOMAIN-3 BY ESCHERICHIA-COLI AND ALTERATION OF INHIBITORY SPECIFICITY TOWARD PROTEASES BY SUBSTITUTION OF THE P1 SITE RESIDUE

S KOJIMA, N FUSHIMI, A IKEDA, KUMAGAI, I, K MIURA

GENE 143(2) 239-243 1994年6月
ACHIEVEMENT OF RENATURATION OF SUBTILISIN BPN' BY A NOVEL PROCEDURE USING ORGANIC SALTS AND A DIGESTIBLE MUTANT OF STREPTOMYCES SUBTILISIN INHIBITOR

M MATSUBARA, E KURIMOTO, S KOJIMA, K MIURA, T SAKAI

FEBS LETTERS 342(2) 193-196 1994年4月
IMPROVEMENT OF A USEFUL ENZYME (SUBTILISIN BPN') BY AN EXPERIMENTAL EVOLUTION SYSTEM

T TANGE, S TAGUCHI, S KOJIMA, K MIURA, H MOMOSE

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 41(2) 239-244 1994年4月
COMPARATIVE-STUDIES ON THE PRIMARY STRUCTURES AND INHIBITORY PROPERTIES OF SUBTILISIN-TRYPSIN INHIBITORS FROM STREPTOMYCES

S TAGUCHI, S KOJIMA, M TERABE, K MIURA, H MOMOSE

EUROPEAN JOURNAL OF BIOCHEMISTRY 220(3) 911-918 1994年3月
NOVEL FMN-BINDING PROTEIN FROM DESULFOVIBRIO-VULGARIS (MIYAZAKI-F) - CLONING AND EXPRESSION OF ITS GENE IN ESCHERICHIA-COLI

M KITAMURA, S KOJIMA, K OGASAWARA, T NAKAYA, T SAGARA, K NIKI, K MIURA, H AKUTSU, KUMAGAI, I

JOURNAL OF BIOLOGICAL CHEMISTRY 269(8) 5566-5573 1994年2月
THE AMINO-ACID-SEQUENCE AND PHOSPHORYLATION SITES OF A BOAR TRANSITION PROTEIN-1

K AKAMA, S KOJIMA, M NAKANO, T TOBITA, H HAYASHI

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 32(2) 349-357 1994年2月
Three novel subtilisin-trypsin inhibitors from Streptomyces: Primary structures and inhibitory properties

小島修一, Mahito Terabe, Shuichi Kojima, Seiichi Taguchi, Haruo Momose, Kin-ichiro Miura

The Journal of Biochemistry 116(5) 1156-1163 1994年
CONTRIBUTION OF SALT BRIDGE IN THE PROTEASE INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR) TO ITS INHIBITORY-ACTION

S KOJIMA, K FUJIMURA, KUMAGAI, I, K MIURA

FEBS LETTERS 337(2) 195-199 1994年1月
Comparative studies on the primary structures and inhibitory properties of subtilisin‐trypsin inhibitors from Streptomyces

Seiichi TAGUCHI, Shuichi KOJIMA, Mahito TERABE, Kin‐ichiro MIURA, Haruo MOMOSE

European Journal of Biochemistry 220(3) 911-918 1994年
Primary structure and inhibitory properties of a proteinase inhibitor produced by Streptomyces cacaoi

KOJIMA SHUICHI, Shuichi Kojima, Mahito Terabe, Seiichi Taguchi, Haruo Momose, Kin-ichiro Miura

Biochimica et Biophysica Acta 1207(1) 120-125 1994年
Three novel subtilisin-trypsin inhibitors from Streptomyces: Primary structures and inhibitory properties

Mahito Terabe, Shuichi Kojima, Seiichi Taguchi, Haruo Momose, Kin-Ichiro Miura

Journal of Biochemistry 116(5) 1156-1163 1994年
STREPTOMYCES SUBTILISIN INHIBITOR-LIKE PROTEINS ARE DISTRIBUTED WIDELY IN STREPTOMYCETES

S TAGUCHI, H KIKUCHI, M SUZUKI, S KOJIMA, M TERABE, KI MIURA, T NAKASE, H MOMOSE

APPLIED AND ENVIRONMENTAL MICROBIOLOGY 59(12) 4338-4341 1993年12月
IDENTIFICATION OF AMINO-ACID-RESIDUES RESPONSIBLE FOR THE CHANGES OF ABSORPTION AND FLUORESCENCE-SPECTRA ON THE BINDING OF SUBTILISIN BPN AND STREPTOMYCES SUBTILISIN INHIBITOR

K MASUDAMOMMA, T SHIMAKAWA, K INOUYE, K HIROMI, S KOJIMA, KUMAGAI, I, K MIURA, B TONOMURA

JOURNAL OF BIOCHEMISTRY 114(6) 906-911 1993年12月
INTERACTION OF SUBTILISIN BPN' AND RECOMBINANT STREPTOMYCES SUBTILISIN INHIBITORS WITH SUBSTITUTED-P(1) SITE RESIDUES

K MASUDAMOMMA, T HATANAKA, K INOUYE, K KANAORI, A TAMURA, K AKASAKA, S KOJIMA, KUMAGAI, I, K MIURA, B TONOMURA

JOURNAL OF BIOCHEMISTRY 114(4) 553-559 1993年10月
INTERACTION OF STREPTOMYCES SUBTILISIN INHIBITOR (SSI) WITH STREPTOMYCES-GRISEUS METALLO-ENDOPEPTIDASE-II (SGMP-II)

T KUMAZAKI, K KAJIWARA, S KOJIMA, K MIURA, S ISHII

JOURNAL OF BIOCHEMISTRY 114(4) 570-575 1993年10月
REQUIREMENT FOR A DISULFIDE BRIDGE NEAR THE REACTIVE SITE OF PROTEASE INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR) FOR ITS INHIBITORY-ACTION

S KOJIMA, KUMAGAI, I, K MIURA

JOURNAL OF MOLECULAR BIOLOGY 230(2) 395-399 1993年3月
HIGH-FREQUENCY OF SSI-LIKE PROTEASE INHIBITORS AMONG STREPTOMYCES

S TAGUCHI, H KIKUCHI, S KOJIMA, KUMAGAI, I, T NAKASE, K MIURA, H MOMOSE

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 57(3) 522-524 1993年3月
Ouantitative in vitro renaturation of subtilisin BPN' without the aid of pro-sequence

小島修一, Mamoru Matsubara, Eiji Kurimito, Shuichi Kojima, Kin-ichiro Miura, Tomoya Sakai

Chemistry Letters 1993(10) 1783-1786 1993年
The primary structure of pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) as determined by nucleotide sequencing of its gene and partial amino acid suquencing

小島修一, Masaya Kitamura, Kiyoshi Ozawa, Shuichi Kojima, Izumi Kumagai, Hideo Akutsu, Kin-ichiro Miura

Protein Sequences and Data Analysis 6(3) 193-196 1993年
Effect of deletion in the flexible loop of the protease inhibitor SSI (Streptomyces subtilisin inhibitor) on Interactions with Proteases

小島修一, Shuichi Kojima, Susumu Furukubo, Izumi Kumagai, Kin-ichro Miura

Protein Engineering 6(3) 207-303 1993年
Ouantitative in vitro renaturation of subtilisin BPN' without the aid of pro-sequence

KOJIMA SHUICHI, Mamoru Matsubara, Eiji Kurimito, Shuichi Kojima, Kin-ichiro Miura, Tomoya Sakai

Chemistry Letters 1993(10) 1783-1786 1993年
The primary structure of pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) as determined by nucleotide sequencing of its gene and partial amino acid suquencing

KOJIMA SHUICHI, Masaya Kitamura, Kiyoshi Ozawa, Shuichi Kojima, Izumi Kumagai, Hideo Akutsu, Kin-ichiro Miura

Protein Sequences and Data Analysis 6(3) 193-196 1993年
Effect of deletion in the flexible loop of the protease inhibitor SSI (Streptomyces subtilisin inhibitor) on Interactions with Proteases

KOJIMA SHUICHI, Shuichi Kojima, Susumu Furukubo, Izumi Kumagai, Kin-ichro Miura

Protein Engineering 6(3) 207-303 1993年
ISOLATION AND PARTIAL CHARACTERIZATION OF SSI-LIKE PROTEASE INHIBITORS FROM STREPTOMYCES

S TAGUCHI, S KOJIMA, KUMAGAI, I, H OGAWARA, K MIURA, H MOMOSE

FEMS MICROBIOLOGY LETTERS 99(2-3) 293-297 1992年12月
A PROTEASE INHIBITOR PRODUCED BY STREPTOMYCES-LIVIDANS 66 EXHIBITS INHIBITORY ACTIVITIES TOWARD BOTH SUBTILISIN BPN' AND TRYPSIN

Y UEDA, S KOJIMA, K TSUMOTO, S TAKEDA, K MIURA, KUMAGAI, I

JOURNAL OF BIOCHEMISTRY 112(2) 204-211 1992年8月
CRYSTAL-STRUCTURE OF AN ENGINEERED SUBTILISIN INHIBITOR COMPLEXES WITH BOVINE TRYPSIN

Y TAKEUCHI, T NONAKA, KT NAKAMURA, S KOJIMA, K MIURA, Y MITSUI

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89(10) 4407-4411 1992年5月
MOLECULAR RECOGNITION AT THE ACTIVE-SITE OF SUBTILISIN BPN' - CRYSTALLOGRAPHIC STUDIES USING GENETICALLY ENGINEERED PROTEINACEOUS INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR)

Y TAKEUCHI, S NOGUCHI, Y SATOW, S KOJIMA, KUMAGAI, I, K MIURA, KT NAKAMURA, Y MITSUI

PROTEIN ENGINEERING 4(5) 501-508 1991年6月
MECHANISMS OF TEMPORARY INHIBITION IN STREPTOMYCES SUBTILISIN INHIBITOR INDUCED BY AN AMINO-ACID SUBSTITUTION, TRYPTOPHAN-86 REPLACED BY HISTIDINE

A TAMURA, K KANAORI, S KOJIMA, KUMAGAI, I, K MIURA, K AKASAKA

BIOCHEMISTRY 30(21) 5275-5286 1991年5月
INHIBITION OF SUBTILISIN BPN' BY REACTION SITE-P1 MUTANTS OF STREPTOMYCES SUBTILISIN INHIBITOR

S KOJIMA, Y NISHIYAMA, KUMAGAI, I, KI MIURA

JOURNAL OF BIOCHEMISTRY 109(3) 377-382 1991年3月
EFFECT ON INHIBITORY ACTIVITY OF MUTATION AT REACTION SITE-P4 OF THE STREPTOMYCES SUBTILISIN INHIBITOR, SSI

S KOJIMA, KUMAGAI, I, KI MIURA

PROTEIN ENGINEERING 3(6) 527-530 1990年5月
ALTERATION OF THE SPECIFICITY OF THE STREPTOMYCES SUBTILISIN INHIBITOR BY GENE ENGINEERING

S KOJIMA, S OBATA, KUMAGAI, I, K MIURA

BIO-TECHNOLOGY 8(5) 449-452 1990年5月
Partial alteration of a protein Streptomyces subtilisin inhibitor by gene engineering

小島修一, Kin-ichiro, Izumi Kumagai, Shusei Obata, Shuichi Kojima, Seiichi Taguchi

Proceedings of the Japan Academy 64B(6) 147-149 1988年
Partial alteration of a protein Streptomyces subtilisin inhibitor by gene engineering

KOJIMA SHUICHI, Kin-ichiro, Izumi Kumagai, Shusei Obata, Shuichi Kojima, Seiichi Taguchi

Proceedings of the Japan Academy 64B(6) 147-149 1988年
CONVERSION OF TRP-62 OF HEN EGG-WHITE LYSOZYME TO TYR BY SITE-DIRECTED MUTAGENESIS

KUMAGAI, I, S KOJIMA, E TAMAKI, K MIURA

JOURNAL OF BIOCHEMISTRY 102(4) 733-740 1987年10月
Characterization of cysteinyl sulfhydryls and nuclease sensitivity of boar sperm nuclei during epididymal maturation

小島修一, Tohru Tobita, Hiroshi Tanaka, Katsuo Tanaka, Shuichi Kojima, Minoru Nakano

Biochemistry International 9(2) 161-168 1984年
Characterization of cysteinyl sulfhydryls and nuclease sensitivity of boar sperm nuclei during epididymal maturation

KOJIMA SHUICHI, Tohru Tobita, Hiroshi Tanaka, Katsuo Tanaka, Shuichi Kojima, Minoru Nakano

Biochemistry International 9(2) 161-168 1984年

書籍等出版物

廣川タンパク質化学第7巻制御タンパク質‐インヒビター 36ズブチリシンインヒビター‐（共著）

廣川書店 2002年
プロペチドおよびプロテアーゼによるプロテアーゼのフォールディング

蛋白質・核酸・酵素 2001年
プロテアーゼのプロペプチドの二面性:インヒビターおよび分子シャペロン

バイオサイエンスとインダストリー 1998年
シリーズ分子生物学6 構造生物学 4.6 プロテアーゼとインヒビター

朝倉書店 1998年
バイオテクノロジー教科書シリーズ9 蛋白質工学概論 (共著)

株式会社コロナ社 1995年

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L-アミノ酸連結酵素の結晶構造解析

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抗生物質バチライシン生合成酵素の結晶構造解析

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第7回日本蛋白質科学会年会 2007年

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所属学協会

共同研究・競争的資金等の研究課題

抗生物質の生合成に関わる酵素の構造と機能に関する研究

2009年
Relationship of the protease inhibitor and molecular chaperon activities in the propeptides

Grant-in-Aid for Scientific Research 1996年
プロペプチドおよびプロテアーゼインヒビターによる蛋白質の立体構造形成補助作用に関する研究

科学研究費補助金 1996年
Synthesis and Characterization of α-Helix-forming Peptide

Grant-in-Aid for Scientific Research 1989年
αヘリックス形成ペプチドによる集合体形成の分子機構に関する研究

科学研究費補助金 1989年

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一覧へ戻る

小島 修一

基本情報

研究キーワード

研究分野

経歴

学歴

MISC

書籍等出版物

講演・口頭発表等

所属学協会

共同研究・競争的資金等の研究課題

小島修一