Shuichi Kojima

(小島修一)

Profile Information

Affiliation: Professor, Faculty of Science, Department of Life Science, Faculty of Science Department of Life Science, Gakushuin University

Degree: 理学士(千葉大学)
(BLANK)(Chiba University)
(BLANK)(The University of Tokyo)

J-GLOBAL ID: 200901087624745392
researchmap Member ID: 1000068615

Research Interests

Research Areas

Research History

1991 - 2007

:学習院大学理学部助教授
1991 - 2007

:Associate Professor, Faculty of Science, Gakushuin University
2007

-:学習院大学理学部教授
2007

-:Professor, Faculty of Science, Gakushuin University
1989 - 1991

:東京大学工学部助手

Education

Misc.

100

New subtilisin-trypsin inhibitors produced by Streptomyces: Primary structures and their relationship to other proteinase inhibitors from Streptomyces

M Terabe, S Kojima, S Taguchi, H Momose, K Miura

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1292(2) 233-240, Feb, 1996
Taxonomic characterization of closely related Streptomyces spp Based on the amino acid sequence analysis of protease inhibitor proteins

S Taguchi, S Kojima, K Miura, H Momose

FEMS MICROBIOLOGY LETTERS, 135(2-3) 169-173, Jan, 1996
Dynamics of the three methionyl side chains of Streptomyces subtilisin inhibitor. Deuterium NMR studies in solution and in the solid state

A Tamura, M Matsushita, A Naito, S Kojima, KI Miura, K Akasaka

PROTEIN SCIENCE, 5(1) 127-139, Jan, 1996
STREPTOMYCES SERINE-PROTEASE (SAM-P20) - RECOMBINANT PRODUCTION, CHARACTERIZATION, AND INTERACTION WITH ENDOGENOUS PROTEASE INHIBITOR

S TAGUCHI, M SUZUKI, S KOJIMA, K MIURA, H MOMOSE

JOURNAL OF BACTERIOLOGY, 177(22) 6638-6643, Nov, 1995
THE AMINO-ACID-SEQUENCE AND INTERACTION WITH THE NUCLEOSOME CORE DNA OF TRANSITION PROTEIN 4 FROM BOAR LATE SPERMATID NUCLEI

K AKAMA, H ICHIMURA, H SATO, S KOJIMA, KI MIURA, H HAYASHI, Y KOMATSU, M NAKANO

EUROPEAN JOURNAL OF BIOCHEMISTRY, 233(1) 179-185, Oct, 1995
A SUBTILISIN INHIBITOR PRODUCED BY STREPTOMYCES-BIKINIENSIS POSSESSES A GLUTAMINE RESIDUE AT REACTIVE-SITE P1

M TERABE, S KOJIMA, S TAGUCHI, H MOMOSE, K MIURA

JOURNAL OF BIOCHEMISTRY, 117(3) 609-613, Mar, 1995
A thermodynamic study of mutant forms of Streptomyces subtilisin inhibitor. II. Replacements at the interface of dimer formation, Val13

小島修一, Atsuo Tamura, Shuichi Kojima, Kin-ichro Miura, Julian M. Sturtevant

Journal of Molecular Biology, 249(3) 636-645, 1995
A subtilisin inhibitor produced by Streptomyces bikiniensis possesses a glutamine residue at reactive site P1

KOJIMA SHUICHI, Mahito Terabe, Shuichi Kojima, Seiichi Taguchi, Haruo Momose, Kin-ichiro Miura

The Journal of Biochemistry, 117(3) 609-613, 1995
A thermodynamic study of mutant forms of Streptomyces subtilisin inhibitor. II. Replacements at the interface of dimer formation, Val13

KOJIMA SHUICHI, Atsuo Tamura, Shuichi Kojima, Kin-ichro Miura, Julian M. Sturtevant

Journal of Molecular Biology, 249(3) 636-645, 1995
Streptomyces serine protease (SAM-P20): Recombinant production,characterization, and interaction with endogenous protease inhibitor

KOJIMA SHUICHI, Seiichi Taguchi, Masayuki Suzuki, Shuichi Kojima, Kin-ichiro Miura, Haruo Momose

Journal of Bacteriology, 177(22) 6638-6643, 1995
PRIMARY STRUCTURE AND INHIBITORY PROPERTIES OF A SUBTILISIN-CHYMOTRYPSIN INHIBITOR FROM STREPTOMYCES-VIRGINIAE

M TERABE, S KOJIMA, S TAGUCHI, H MOMOSE, K MIURA

EUROPEAN JOURNAL OF BIOCHEMISTRY, 226(2) 627-632, Dec, 1994
EFFECT OF AN INTERSUBUNIT DISULFIDE BOND ON THE STABILITY OF STREPTOMYCES SUBTILISIN INHIBITOR

A TAMURA, S KOJIMA, K MIURA, JM STURTEVANT

BIOCHEMISTRY, 33(48) 14512-14520, Dec, 1994
RENATURATION OF THE MATURE SUBTILISIN BPN' IMMOBILIZED ON AGAROSE BEADS

T HAYASHI, M MATSUBARA, D NOHARA, S KOJIMA, K MIURA, T SAKAI

FEBS LETTERS, 350(1) 109-112, Aug, 1994
PRIMARY STRUCTURE AND INHIBITORY PROPERTIES OF A PROTEINASE-INHIBITOR PRODUCED BY STREPTOMYCES-CACAOI

S KOJIMA, M TERABE, S TAGUCHI, H MOMOSE, KI MIURA

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1207(1) 120-125, Jul, 1994
SECRETORY PRODUCTION OF CHICKEN OVOMUCOID DOMAIN-3 BY ESCHERICHIA-COLI AND ALTERATION OF INHIBITORY SPECIFICITY TOWARD PROTEASES BY SUBSTITUTION OF THE P1 SITE RESIDUE

S KOJIMA, N FUSHIMI, A IKEDA, KUMAGAI, I, K MIURA

GENE, 143(2) 239-243, Jun, 1994
ACHIEVEMENT OF RENATURATION OF SUBTILISIN BPN' BY A NOVEL PROCEDURE USING ORGANIC SALTS AND A DIGESTIBLE MUTANT OF STREPTOMYCES SUBTILISIN INHIBITOR

M MATSUBARA, E KURIMOTO, S KOJIMA, K MIURA, T SAKAI

FEBS LETTERS, 342(2) 193-196, Apr, 1994
IMPROVEMENT OF A USEFUL ENZYME (SUBTILISIN BPN') BY AN EXPERIMENTAL EVOLUTION SYSTEM

T TANGE, S TAGUCHI, S KOJIMA, K MIURA, H MOMOSE

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 41(2) 239-244, Apr, 1994
COMPARATIVE-STUDIES ON THE PRIMARY STRUCTURES AND INHIBITORY PROPERTIES OF SUBTILISIN-TRYPSIN INHIBITORS FROM STREPTOMYCES

S TAGUCHI, S KOJIMA, M TERABE, K MIURA, H MOMOSE

EUROPEAN JOURNAL OF BIOCHEMISTRY, 220(3) 911-918, Mar, 1994
NOVEL FMN-BINDING PROTEIN FROM DESULFOVIBRIO-VULGARIS (MIYAZAKI-F) - CLONING AND EXPRESSION OF ITS GENE IN ESCHERICHIA-COLI

M KITAMURA, S KOJIMA, K OGASAWARA, T NAKAYA, T SAGARA, K NIKI, K MIURA, H AKUTSU, KUMAGAI, I

JOURNAL OF BIOLOGICAL CHEMISTRY, 269(8) 5566-5573, Feb, 1994
THE AMINO-ACID-SEQUENCE AND PHOSPHORYLATION SITES OF A BOAR TRANSITION PROTEIN-1

K AKAMA, S KOJIMA, M NAKANO, T TOBITA, H HAYASHI

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 32(2) 349-357, Feb, 1994
Three novel subtilisin-trypsin inhibitors from Streptomyces: Primary structures and inhibitory properties

小島修一, Mahito Terabe, Shuichi Kojima, Seiichi Taguchi, Haruo Momose, Kin-ichiro Miura

The Journal of Biochemistry, 116(5) 1156-1163, 1994
CONTRIBUTION OF SALT BRIDGE IN THE PROTEASE INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR) TO ITS INHIBITORY-ACTION

S KOJIMA, K FUJIMURA, KUMAGAI, I, K MIURA

FEBS LETTERS, 337(2) 195-199, Jan, 1994
Comparative studies on the primary structures and inhibitory properties of subtilisin‐trypsin inhibitors from Streptomyces

Seiichi TAGUCHI, Shuichi KOJIMA, Mahito TERABE, Kin‐ichiro MIURA, Haruo MOMOSE

European Journal of Biochemistry, 220(3) 911-918, 1994
Primary structure and inhibitory properties of a proteinase inhibitor produced by Streptomyces cacaoi

KOJIMA SHUICHI, Shuichi Kojima, Mahito Terabe, Seiichi Taguchi, Haruo Momose, Kin-ichiro Miura

Biochimica et Biophysica Acta, 1207(1) 120-125, 1994
Three novel subtilisin-trypsin inhibitors from Streptomyces: Primary structures and inhibitory properties

Mahito Terabe, Shuichi Kojima, Seiichi Taguchi, Haruo Momose, Kin-Ichiro Miura

Journal of Biochemistry, 116(5) 1156-1163, 1994
STREPTOMYCES SUBTILISIN INHIBITOR-LIKE PROTEINS ARE DISTRIBUTED WIDELY IN STREPTOMYCETES

S TAGUCHI, H KIKUCHI, M SUZUKI, S KOJIMA, M TERABE, KI MIURA, T NAKASE, H MOMOSE

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 59(12) 4338-4341, Dec, 1993
IDENTIFICATION OF AMINO-ACID-RESIDUES RESPONSIBLE FOR THE CHANGES OF ABSORPTION AND FLUORESCENCE-SPECTRA ON THE BINDING OF SUBTILISIN BPN AND STREPTOMYCES SUBTILISIN INHIBITOR

K MASUDAMOMMA, T SHIMAKAWA, K INOUYE, K HIROMI, S KOJIMA, KUMAGAI, I, K MIURA, B TONOMURA

JOURNAL OF BIOCHEMISTRY, 114(6) 906-911, Dec, 1993
INTERACTION OF SUBTILISIN BPN' AND RECOMBINANT STREPTOMYCES SUBTILISIN INHIBITORS WITH SUBSTITUTED-P(1) SITE RESIDUES

K MASUDAMOMMA, T HATANAKA, K INOUYE, K KANAORI, A TAMURA, K AKASAKA, S KOJIMA, KUMAGAI, I, K MIURA, B TONOMURA

JOURNAL OF BIOCHEMISTRY, 114(4) 553-559, Oct, 1993
INTERACTION OF STREPTOMYCES SUBTILISIN INHIBITOR (SSI) WITH STREPTOMYCES-GRISEUS METALLO-ENDOPEPTIDASE-II (SGMP-II)

T KUMAZAKI, K KAJIWARA, S KOJIMA, K MIURA, S ISHII

JOURNAL OF BIOCHEMISTRY, 114(4) 570-575, Oct, 1993
REQUIREMENT FOR A DISULFIDE BRIDGE NEAR THE REACTIVE SITE OF PROTEASE INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR) FOR ITS INHIBITORY-ACTION

S KOJIMA, KUMAGAI, I, K MIURA

JOURNAL OF MOLECULAR BIOLOGY, 230(2) 395-399, Mar, 1993
HIGH-FREQUENCY OF SSI-LIKE PROTEASE INHIBITORS AMONG STREPTOMYCES

S TAGUCHI, H KIKUCHI, S KOJIMA, KUMAGAI, I, T NAKASE, K MIURA, H MOMOSE

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 57(3) 522-524, Mar, 1993
Ouantitative in vitro renaturation of subtilisin BPN' without the aid of pro-sequence

小島修一, Mamoru Matsubara, Eiji Kurimito, Shuichi Kojima, Kin-ichiro Miura, Tomoya Sakai

Chemistry Letters, 1993(10) 1783-1786, 1993
The primary structure of pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) as determined by nucleotide sequencing of its gene and partial amino acid suquencing

小島修一, Masaya Kitamura, Kiyoshi Ozawa, Shuichi Kojima, Izumi Kumagai, Hideo Akutsu, Kin-ichiro Miura

Protein Sequences and Data Analysis, 6(3) 193-196, 1993
Effect of deletion in the flexible loop of the protease inhibitor SSI (Streptomyces subtilisin inhibitor) on Interactions with Proteases

小島修一, Shuichi Kojima, Susumu Furukubo, Izumi Kumagai, Kin-ichro Miura

Protein Engineering, 6(3) 207-303, 1993
Ouantitative in vitro renaturation of subtilisin BPN' without the aid of pro-sequence

KOJIMA SHUICHI, Mamoru Matsubara, Eiji Kurimito, Shuichi Kojima, Kin-ichiro Miura, Tomoya Sakai

Chemistry Letters, 1993(10) 1783-1786, 1993
The primary structure of pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) as determined by nucleotide sequencing of its gene and partial amino acid suquencing

KOJIMA SHUICHI, Masaya Kitamura, Kiyoshi Ozawa, Shuichi Kojima, Izumi Kumagai, Hideo Akutsu, Kin-ichiro Miura

Protein Sequences and Data Analysis, 6(3) 193-196, 1993
Effect of deletion in the flexible loop of the protease inhibitor SSI (Streptomyces subtilisin inhibitor) on Interactions with Proteases

KOJIMA SHUICHI, Shuichi Kojima, Susumu Furukubo, Izumi Kumagai, Kin-ichro Miura

Protein Engineering, 6(3) 207-303, 1993
ISOLATION AND PARTIAL CHARACTERIZATION OF SSI-LIKE PROTEASE INHIBITORS FROM STREPTOMYCES

S TAGUCHI, S KOJIMA, KUMAGAI, I, H OGAWARA, K MIURA, H MOMOSE

FEMS MICROBIOLOGY LETTERS, 99(2-3) 293-297, Dec, 1992
A PROTEASE INHIBITOR PRODUCED BY STREPTOMYCES-LIVIDANS 66 EXHIBITS INHIBITORY ACTIVITIES TOWARD BOTH SUBTILISIN BPN' AND TRYPSIN

Y UEDA, S KOJIMA, K TSUMOTO, S TAKEDA, K MIURA, KUMAGAI, I

JOURNAL OF BIOCHEMISTRY, 112(2) 204-211, Aug, 1992
CRYSTAL-STRUCTURE OF AN ENGINEERED SUBTILISIN INHIBITOR COMPLEXES WITH BOVINE TRYPSIN

Y TAKEUCHI, T NONAKA, KT NAKAMURA, S KOJIMA, K MIURA, Y MITSUI

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 89(10) 4407-4411, May, 1992
MOLECULAR RECOGNITION AT THE ACTIVE-SITE OF SUBTILISIN BPN' - CRYSTALLOGRAPHIC STUDIES USING GENETICALLY ENGINEERED PROTEINACEOUS INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR)

Y TAKEUCHI, S NOGUCHI, Y SATOW, S KOJIMA, KUMAGAI, I, K MIURA, KT NAKAMURA, Y MITSUI

PROTEIN ENGINEERING, 4(5) 501-508, Jun, 1991
MECHANISMS OF TEMPORARY INHIBITION IN STREPTOMYCES SUBTILISIN INHIBITOR INDUCED BY AN AMINO-ACID SUBSTITUTION, TRYPTOPHAN-86 REPLACED BY HISTIDINE

A TAMURA, K KANAORI, S KOJIMA, KUMAGAI, I, K MIURA, K AKASAKA

BIOCHEMISTRY, 30(21) 5275-5286, May, 1991
INHIBITION OF SUBTILISIN BPN' BY REACTION SITE-P1 MUTANTS OF STREPTOMYCES SUBTILISIN INHIBITOR

S KOJIMA, Y NISHIYAMA, KUMAGAI, I, KI MIURA

JOURNAL OF BIOCHEMISTRY, 109(3) 377-382, Mar, 1991
EFFECT ON INHIBITORY ACTIVITY OF MUTATION AT REACTION SITE-P4 OF THE STREPTOMYCES SUBTILISIN INHIBITOR, SSI

S KOJIMA, KUMAGAI, I, KI MIURA

PROTEIN ENGINEERING, 3(6) 527-530, May, 1990
ALTERATION OF THE SPECIFICITY OF THE STREPTOMYCES SUBTILISIN INHIBITOR BY GENE ENGINEERING

S KOJIMA, S OBATA, KUMAGAI, I, K MIURA

BIO-TECHNOLOGY, 8(5) 449-452, May, 1990
Partial alteration of a protein Streptomyces subtilisin inhibitor by gene engineering

小島修一, Kin-ichiro, Izumi Kumagai, Shusei Obata, Shuichi Kojima, Seiichi Taguchi

Proceedings of the Japan Academy, 64B(6) 147-149, 1988
Partial alteration of a protein Streptomyces subtilisin inhibitor by gene engineering

KOJIMA SHUICHI, Kin-ichiro, Izumi Kumagai, Shusei Obata, Shuichi Kojima, Seiichi Taguchi

Proceedings of the Japan Academy, 64B(6) 147-149, 1988
CONVERSION OF TRP-62 OF HEN EGG-WHITE LYSOZYME TO TYR BY SITE-DIRECTED MUTAGENESIS

KUMAGAI, I, S KOJIMA, E TAMAKI, K MIURA

JOURNAL OF BIOCHEMISTRY, 102(4) 733-740, Oct, 1987
Characterization of cysteinyl sulfhydryls and nuclease sensitivity of boar sperm nuclei during epididymal maturation

小島修一, Tohru Tobita, Hiroshi Tanaka, Katsuo Tanaka, Shuichi Kojima, Minoru Nakano

Biochemistry International, 9(2) 161-168, 1984
Characterization of cysteinyl sulfhydryls and nuclease sensitivity of boar sperm nuclei during epididymal maturation

KOJIMA SHUICHI, Tohru Tobita, Hiroshi Tanaka, Katsuo Tanaka, Shuichi Kojima, Minoru Nakano

Biochemistry International, 9(2) 161-168, 1984

Books and Other Publications

廣川タンパク質化学第7巻制御タンパク質‐インヒビター 36ズブチリシンインヒビター‐（共著）

廣川書店, 2002
プロペチドおよびプロテアーゼによるプロテアーゼのフォールディング

蛋白質・核酸・酵素, 2001
プロテアーゼのプロペプチドの二面性:インヒビターおよび分子シャペロン

バイオサイエンスとインダストリー, 1998
シリーズ分子生物学6 構造生物学 4.6 プロテアーゼとインヒビター

朝倉書店, 1998
バイオテクノロジー教科書シリーズ9 蛋白質工学概論 (共著)

株式会社コロナ社, 1995

Presentations

L-アミノ酸連結酵素YwfEの基質認識様式

第85回日本生化学会大会, 2012
L-アミノ酸連結酵素の結晶構造解析

第11回日本蛋白質科学会年会, 2011
抗生物質バチライシン生合成酵素の結晶構造解析

第84回日本生化学会大会, 2011
銅イオンポンプのドメイン間相互作用

第82回日本生化学会大会, 2009
分子内シャペロンとしてのPOIA1

第7回日本蛋白質科学会年会, 2007

Professional Memberships

Research Projects

抗生物質の生合成に関わる酵素の構造と機能に関する研究

2009
Relationship of the protease inhibitor and molecular chaperon activities in the propeptides

Grant-in-Aid for Scientific Research, 1996
プロペプチドおよびプロテアーゼインヒビターによる蛋白質の立体構造形成補助作用に関する研究

科学研究費補助金, 1996
Synthesis and Characterization of α-Helix-forming Peptide

Grant-in-Aid for Scientific Research, 1989
αヘリックス形成ペプチドによる集合体形成の分子機構に関する研究

科学研究費補助金, 1989

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