Shuichi Kojima

(小島修一)

Profile Information

Affiliation: Professor, Faculty of Science, Department of Life Science, Faculty of Science Department of Life Science, Gakushuin University

Degree: 理学士(千葉大学)
(BLANK)(Chiba University)
(BLANK)(The University of Tokyo)

J-GLOBAL ID: 200901087624745392
researchmap Member ID: 1000068615

Research Interests

Research Areas

Research History

1991 - 2007

:学習院大学理学部助教授
1991 - 2007

:Associate Professor, Faculty of Science, Gakushuin University
2007

-:学習院大学理学部教授
2007

-:Professor, Faculty of Science, Gakushuin University
1989 - 1991

:東京大学工学部助手

Education

Misc.

100

pH responsiveness of fibrous assemblies of repeat-sequence amphipathic alpha-helix polypeptides

Toshiaki Takei, Kouhei Tsumoto, Atsuhito Okonogi, Akiko Kimura, Shuichi Kojima, Kazumori Yazaki, Tsunetomo Takei, Takuya Ueda, Kin-ichiro Miura

PROTEIN SCIENCE, 24(5) 883-894, May, 2015
Role of Positions e and g in the Fibrous Assembly Formation of an Amphipathic alpha-Helix-Forming Polypeptide

Toshiaki Takei, Kouhei Tsumoto, Masakuni Yoshino, Shuichi Kojima, Kazumori Yazaki, Takuya Ueda, Tsunetomo Takei, Fumio Arisaka, Kin-ichiro Miura

BIOPOLYMERS, 102(3) 260-272, May, 2014
Single Mutation Alters the Substrate Specificity of L-Amino Acid Ligase

Takeo Tsuda, Mana Asami, Yoshiaki Koguchi, Shuichi Kojima

BIOCHEMISTRY, 53(16) 2650-2660, Apr, 2014
Effects of Chain Length of an Amphipathic Polypeptide Carrying the Repeated Amino Acid Sequence (LETLAKA)(n) on alpha-Helix and Fibrous Assembly Formation

Toshiaki Takei, Kazuya Hasegawa, Katsumi Imada, Keiichi Namba, Kouhei Tsumoto, Yukino Kuriki, Masakuni Yoshino, Kazumori Yazaki, Shuichi Kojima, Tsunetomo Takei, Takuya Ueda, Kin-ichiro Miura

BIOCHEMISTRY, 52(16) 2810-2820, Apr, 2013
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an l-amino-acid ligase

Takeo Tsuda, Tomomi Suzuki, Shuichi Kojima

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 68(2) 203-206, Feb, 2012
Increasing the hydrolysis constant of the reactive site upon introduction of an engineered Cys(14)-Cys(39) bond into the ovomucoid third domain from silver pheasant

Hikaru Hemmi, Takashi Kumazaki, Shuichi Kojima, Takuya Yoshida, Tadayasu Ohkubo, Hideyoshi Yokosawa, Kin-ichiro Miura, Yuji Kobayashi

JOURNAL OF PEPTIDE SCIENCE, 17(8) 595-600, Aug, 2011
Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family

Naoki Shibata, Yasufumi Ueda, Daisuke Takeuchi, Yoshihiro Haruyama, Shuichi Kojima, Junichi Sato, Youichi Niimura, Masaya Kitamura, Yoshiki Higuchi

FEBS JOURNAL, 276(17) 4840-4853, Sep, 2009
Requirement of Ala residues at g position in heptad sequence of alpha-helix-forming peptide for formation of fibrous structure

Gaku Aoki, Toyo K. Yamada, Mayu Arii, Shuichi Kojima, Tadashi Mizoguchi

JOURNAL OF BIOCHEMISTRY, 144(1) 15-19, Jul, 2008
Requirement of Ala residues at g position in heptad sequence of alpha-helix-forming peptide for formation of fibrous structure

Gaku Aoki, Toyo K. Yamada, Mayu Arii, Shuichi Kojima, Tadashi Mizoguchi

JOURNAL OF BIOCHEMISTRY, 144(1) 15-19, Jul, 2008
Effects of hydrophobic amino acid substitution in Pleurotus ostreatus proteinase A inhibitor 1 on its structure and functions as protease inhibitor and intramolecular chaperone

Shuichi Kojima, Akane Iwahara, Yuri Hisano, Hideyuki Yanai

PROTEIN ENGINEERING DESIGN & SELECTION, 20(5) 211-217, May, 2007
The effects of the side chains of hydrophobic aliphatic amino acid residues in an amphipathic polypeptide on the formation of a helix and its association

T Takei, A Okonogi, K Tateno, A Kimura, S Kojima, K Yazaki, K Miura

JOURNAL OF BIOCHEMISTRY, 139(2) 271-278, Feb, 2006
The effects of the side chains of hydrophobic aliphatic amino acid residues in an amphipathic polypeptide on the formation of a helix and its association

T Takei, A Okonogi, K Tateno, A Kimura, S Kojima, K Yazaki, K Miura

JOURNAL OF BIOCHEMISTRY, 139(2) 271-278, Feb, 2006
The structure of the third intracellular loop of the muscarinic acetylcholine receptor M-2 subtype

S Ichlyama, Y Oka, K Haga, S Kojima, Y Tateishi, M Shirakawa, T Haga

FEBS LETTERS, 580(1) 23-26, Jan, 2006
Inhibitor-assisted refolding of protease: A protease inhibitor as an intramolecular chaperone

S Kojima, A Iwahara, H Yanai

FEBS LETTERS, 579(20) 4430-4436, Aug, 2005
Stabilization of the fibrous structure of an alpha-helix-forming peptide by sequence reversal

S Kojima, Y Kuriki, K Yazaki, K Miura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 331(2) 577-582, Jun, 2005
Stabilization of the fibrous structure of an alpha-helix-forming peptide by sequence reversal

S Kojima, Y Kuriki, K Yazaki, K Miura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 331(2) 577-582, Jun, 2005
Mapping the position of the transition state in the folding of a small alpha/beta protein, POIA1

S Tanaka, S Kojima, A Tamura

CHEMICAL PHYSICS, 307(2-3) 233-242, Dec, 2004
Mapping the position of the transition state in the folding of a small alpha/beta protein, POIA1

S Tanaka, S Kojima, A Tamura

CHEMICAL PHYSICS, 307(2-3) 233-242, Dec, 2004
Low pH facilitates uptake of proteins by cells through a non-endocytic pathway

M Motizuki, T Takei, K Tasaka, S Yokota, S Kojima, T Haga, K Tsurugi

JOURNAL OF BIOCHEMISTRY, 135(6) 713-719, Jun, 2004
Cloning and expression of the enolase gene from Desulfovibrio vulgaris (Miyazaki F)

M Kitamura, Y Takayama, S Kojima, K Kohno, H Ogata, Y Higuchi, H Inoue

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1676(2) 172-181, Jan, 2004
Inhibitory specificity change of the ovomucoid third domain of the silver pheasant upon introduction of an engineered Cys(14)-Cys(39) bond

H Hemmi, T Kumazaki, T Yamazaki, S Kojima, T Yoshida, Y Kyogoku, M Katsu, F Shinohara, H Yokosawa, K Miura, Y Kobayashi

BIOCHEMISTRY, 42(9) 2524-2534, Mar, 2003
Functional tolerance of Streptomyces subtilisin inhibitor toward conformational and stability changes caused by single-point mutations in the hydrophobic core

M Oda, A Tamura, K Kanaori, S Kojima, K Miura, K Momma, B Tonomura, K Akasaka

JOURNAL OF BIOCHEMISTRY, 132(6) 991-995, Dec, 2002
Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-II

M Motizuki, T Satoh, T Takei, T Itoh, S Yokota, S Kojima, K Miura, T Samejima, K Tsurugi

JOURNAL OF BIOCHEMISTRY, 132(1) 115-119, Jul, 2002
Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-II

M Motizuki, T Satoh, T Takei, T Itoh, S Yokota, S Kojima, K Miura, T Samejima, K Tsurugi

JOURNAL OF BIOCHEMISTRY, 132(1) 115-119, Jul, 2002
Requirement for hydrophobic Phe residues in Pleurotus ostreatus proteinase A inhibitor 1 for stable inhibition

S Kojima, Y Hisano

PROTEIN ENGINEERING, 15(4) 325-329, Apr, 2002
Structure of POIA1, a homologous protein to the propeptide of subtilisin: Implication for protein foldability and the function as an intramolecular chaperone

H Sasakawa, S Yoshinaga, S Kojima, A Tamura

JOURNAL OF MOLECULAR BIOLOGY, 317(1) 159-167, Mar, 2002
Accelerated refolding of subtilisin BPN ' by tertiary-structure-forming mutants of its propeptide

S Kojima, H Yanai, K Miura

JOURNAL OF BIOCHEMISTRY, 130(4) 471-474, Oct, 2001
Cloning and expression of the catalase gene from the anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F)

M Kitamura, T Nakanishi, S Kojima, Kumagai, I, H Inoue

JOURNAL OF BIOCHEMISTRY, 129(3) 357-364, Mar, 2001
Alteration of inhibitory properties of Pleurotus ostreatus proteinase A inhibitor 1 by mutation of its C-terminal region

S Kojima, Y Hisano, K Miura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 281(5) 1271-1276, Mar, 2001
Analysis of the isoform of Xenopus euakryotic translation initiation factor 4E

M Wakiyama, A Suzuki, M Saigoh, N Sakai, H Miyoshi, S Kojima, K Miura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65(1) 232-235, Jan, 2001
Effects of amino acid replacements around the reactive site of chicken ovomucoid domain 3 on the inhibitory activity toward chymotrypsin and trypsin

S Kojima, N Takagi, T Minagawa, N Fushimi, K Miura

PROTEIN ENGINEERING, 12(10) 857-862, Oct, 1999
Expression of a zinc-binding domain of boar spermatidal transition protein 2 in Escherichia coli

H Sato, K Akama, S Kojima, K Miura, A Sekine, M Nakano

PROTEIN EXPRESSION AND PURIFICATION, 16(3) 454-462, Aug, 1999
Involvement of the C-terminal region of yeast proteinase B inhibitor 2 in its inhibitory action

S Kojima, M Deguchi, K Miura

JOURNAL OF MOLECULAR BIOLOGY, 286(3) 775-785, Feb, 1999
High performance in refolding of Streptomyces griseus trypsin by the aid of a mutant of Streptomyces subtilisin inhibitor designed as trypsin inhibitor

D Nohara, H Sugiura, H Sakakibara, M Matsubara, S Kojima, K Miura, T Sakai

JOURNAL OF BIOCHEMISTRY, 125(2) 343-347, Feb, 1999
P1-specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor

T Tanaka, H Matsuzawa, S Kojima, Kumagai, I, K Miura, T Ohta

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62(10) 2035-2038, Oct, 1998
An endogenous target protease, SAM-P26, of Streptomyces protease inhibitor (SSI): Primary structure, enzymatic characterization, and its interaction with SSI

S Taguchi, S Yamada, S Kojima, H Momose

JOURNAL OF BIOCHEMISTRY, 124(4) 804-810, Oct, 1998
Primary structure of Streptomyces griseus metalloendopeptidase II

S Kojima, T Kumazaki, S Ishii, K Miura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 62(7) 1392-1398, Jul, 1998
Cloning and expression of the gene encoding flavodoxin from Desulfovibrio vulgaris (Miyazaki F)

M Kitamura, T Sagara, M Taniguchi, M Ashida, K Ezoe, K Kohno, S Kojima, K Ozawa, H Akutsu, Kumagai, I, T Nakaya

JOURNAL OF BIOCHEMISTRY, 123(5) 891-898, May, 1998
Tertiary structure formation in the propeptide of subtilisin BPN ' by successive amino acid replacements and its close relation to function

S Kojima, T Minagawa, K Miura

JOURNAL OF MOLECULAR BIOLOGY, 277(5) 1007-1013, Apr, 1998
The propeptide of subtilisin BPN' as a temporary inhibitor and effect of an amino acid replacement on its inhibitory activity

S Kojima, T Minagawa, K Miura

FEBS LETTERS, 411(1) 128-132, Jul, 1997
Disulfide bond formation is not involved in cap-binding activity of Xenopus translation initiation factor eIF-4E

Motoaki Wakiyama, Nobuya Sakai, Shuichi Kojima, Kin-Ichiro Miura

FEBS Letters, 409(3) 407-410, Jun 16, 1997
Disulfide bond formation is not involved in cap-binding activity of Xenopus translation initiation factor eIF-4E

M Wakiyama, N Sakai, S Kojima, K Miura

FEBS LETTERS, 409(3) 407-410, Jun, 1997
Molecular phylogenetic characterization of Streptomyces protease inhibitor family

S Taguchi, S Kojima, M Terabe, Y Kumazawa, H Kohriyama, M Suzuki, K Miura, H Momose

JOURNAL OF MOLECULAR EVOLUTION, 44(5) 542-551, May, 1997
Cloning and expression of the rubredoxin gene from Desulfovibrio vulgaris (Miyazaki F) - Comparison of the primary structure of desulfoferrodoxin

M Kitamura, Y Koshino, Y Kamikawa, K Kohno, S Kojima, K Miura, T Sagara, H Akutsu, Kumagai, I, T Nakaya

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1351(1-2) 239-247, Mar, 1997
Fibril formation by an amphipathic alpha-helix-forming polypeptide produced by gene engineering

S Kojima, Y Kuriki, T Yoshida, K Yazaki, K Miura

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 73(1) 7-11, Jan, 1997
A novel member of the subtilisin-like protease family from Streptomyces albogriseolus

M Suzuki, S Taguchi, S Yamada, S Kojima, KI Miura, H Momose

JOURNAL OF BACTERIOLOGY, 179(2) 430-438, Jan, 1997
Fibril formation by an amphipathic alpha-helix-forming polypeptide produced by gene engineering

S Kojima, Y Kuriki, T Yoshida, K Yazaki, K Miura

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 73(1) 7-11, Jan, 1997
Synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity

S Kojima, K Miyoshi, K Miura

PROTEIN ENGINEERING, 9(12) 1241-1246, Dec, 1996
Synthesis of alpha-helix-forming peptides by gene engineering methods and their characterization by circular dichroism spectra measurements

S Kojima, Y Kuriki, Y Sato, F Arisaka, Kumagai, I, S Takahashi, K Miura

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1294(2) 129-137, May, 1996
Synthesis of alpha-helix-forming peptides by gene engineering methods and their characterization by circular dichroism spectra measurements

S Kojima, Y Kuriki, Y Sato, F Arisaka, Kumagai, I, S Takahashi, K Miura

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1294(2) 129-137, May, 1996

Books and Other Publications

廣川タンパク質化学第7巻制御タンパク質‐インヒビター 36ズブチリシンインヒビター‐（共著）

廣川書店, 2002
プロペチドおよびプロテアーゼによるプロテアーゼのフォールディング

蛋白質・核酸・酵素, 2001
プロテアーゼのプロペプチドの二面性:インヒビターおよび分子シャペロン

バイオサイエンスとインダストリー, 1998
シリーズ分子生物学6 構造生物学 4.6 プロテアーゼとインヒビター

朝倉書店, 1998
バイオテクノロジー教科書シリーズ9 蛋白質工学概論 (共著)

株式会社コロナ社, 1995

Presentations

L-アミノ酸連結酵素YwfEの基質認識様式

第85回日本生化学会大会, 2012
L-アミノ酸連結酵素の結晶構造解析

第11回日本蛋白質科学会年会, 2011
抗生物質バチライシン生合成酵素の結晶構造解析

第84回日本生化学会大会, 2011
銅イオンポンプのドメイン間相互作用

第82回日本生化学会大会, 2009
分子内シャペロンとしてのPOIA1

第7回日本蛋白質科学会年会, 2007

Professional Memberships

Research Projects

抗生物質の生合成に関わる酵素の構造と機能に関する研究

2009
Relationship of the protease inhibitor and molecular chaperon activities in the propeptides

Grant-in-Aid for Scientific Research, 1996
プロペプチドおよびプロテアーゼインヒビターによる蛋白質の立体構造形成補助作用に関する研究

科学研究費補助金, 1996
Synthesis and Characterization of α-Helix-forming Peptide

Grant-in-Aid for Scientific Research, 1989
αヘリックス形成ペプチドによる集合体形成の分子機構に関する研究

科学研究費補助金, 1989

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