小島修一

コジマシユウイチ (Shuichi Kojima)

基本情報

所属: 学習院大学理学部生命科学科理学部生命科学科教授

学位: 理学士(千葉大学)
理学修士(千葉大学)
工学博士(東京大学)

J-GLOBAL ID: 200901087624745392
researchmap会員ID: 1000068615

研究キーワード

研究分野

経歴

1991年 - 2007年

:学習院大学理学部助教授
1991年 - 2007年

:Associate Professor, Faculty of Science, Gakushuin University
2007年

-:学習院大学理学部教授
2007年

-:Professor, Faculty of Science, Gakushuin University
1989年 - 1991年

:東京大学工学部助手

もっとみる

学歴

もっとみる

MISC

100

pH resopnsiveness of fibrous assemblies of repeat-sequence amphipathic α-helix polypeptides

Toshiaki Takei, Kouhei Tsumoto, Atsuhiko Okonogi, Akiko Kimura, Shuichi Kojima, Kazumori Yazaki, Tsunetomo Takei, Takuya Ueda, Kin-ichiro Miura

Protein Science 24(5) 883-894 2015年5月
Role of positions e and g in the fibrous assembly formation of an amphipathic α-helix-forming polypeptide

Toshiaki Takei, Kouhei Tsumoto, Masakuni Yoshino, Shuichi Kojima, Kazumori Yazaki, Takuya Ueda, Tsunetomo Takei, Fumio Arisaka, Kin-ichiro Miura

Biopolymers (Peptide Science) 102(3) 260-272 2014年5月
Single Mutation Alters the Substrate Specificity of L-Amino Acid Ligase

Takeo Tsuda, Mana Asami, Yoshiaki Koguchi, Shuichi Kojima

BIOCHEMISTRY 53(16) 2650-2660 2014年4月
Effects of chain length of an amphipathic polypeptide carrying the repeated amino acid sequence (LETLAKA)n on α-helix and fibrous assembly formation

Toshiaki Takei, Kazuya Hasegawa, Katsumi Imada, Keiichi Namba, Kouhei Tsumoto, Yukino Kuriki, Masakuni Yoshino, Kazumori Yazaki, Shuichi Kojima, Tsunetomo Takei, Takuya Ueda, Kin-ichiro

Biochemistry 52(16) 2810-2820 2013年4月
Crystallization and preliminary X-ray diffraction analysis of Bacillus subtilis YwfE, an l-amino-acid ligase

Takeo Tsuda, Tomomi Suzuki, Shuichi Kojima

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 68(2) 203-206 2012年2月
Increasing the hydrolysis constant of the reactive site upon introduction of an engineered Cys(14)-Cys(39) bond into the ovomucoid third domain from silver pheasant

Hikaru Hemmi, Takashi Kumazaki, Shuichi Kojima, Takuya Yoshida, Tadayasu Ohkubo, Hideyoshi Yokosawa, Kin-ichiro Miura, Yuji Kobayashi

JOURNAL OF PEPTIDE SCIENCE 17(8) 595-600 2011年8月
Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family

Naoki Shibata, Yasufumi Ueda, Daisuke Takeuchi, Yoshihiro Haruyama, Shuichi Kojima, Junichi Sato, Youichi Niimura, Masaya Kitamura, Yoshiki Higuchi

FEBS JOURNAL 276(17) 4840-4853 2009年9月
Requirement of Ala residues at g ｐositions in heptad sequence of α-helix-forming peptides for formation of fibrous structure

小島修一, Gaku Aoki, Toyo K. Yamada, Mayu Arii, Shuichi Kojima, Tadashi Mizoguchi

The Journal of Biochemistry 144(1) 15-19 2008年7月
Requirement of Ala residues at g position in heptad sequence of alpha-helix-forming peptide for formation of fibrous structure

Gaku Aoki, Toyo K. Yamada, Mayu Arii, Shuichi Kojima, Tadashi Mizoguchi

JOURNAL OF BIOCHEMISTRY 144(1) 15-19 2008年7月
Effects of hydrophobic amino acid substitution in Pleurotus ostreatus proteinase A inhibitor 1 on its structure and functions as protease inhibitor and intramolecular chaperone

Shuichi Kojima, Akane Iwahara, Yuri Hisano, Hideyuki Yanai

PROTEIN ENGINEERING DESIGN & SELECTION 20(5) 211-217 2007年5月
The effects of the side chains of hydrophobic aliphatic amino acid residues in an amphipathic polypeptide on the formation of α helix and its assocation

小島修一, Toshiaki Takei, Atsuhito Okonogi, Kumiko Tateno, Akiko Kimura, Shuichi Kojima, Kazumori Yazaki. Kin-ichiro Miura

The Journal of Biochemistry 139(2) 271-278 2006年2月
The effects of the side chains of hydrophobic aliphatic amino acid residues in an amphipathic polypeptide on the formation of a helix and its association

T Takei, A Okonogi, K Tateno, A Kimura, S Kojima, K Yazaki, K Miura

JOURNAL OF BIOCHEMISTRY 139(2) 271-278 2006年2月
The structure of the third intracellular loop of the muscarinic acetylcholine receptor M-2 subtype

S Ichlyama, Y Oka, K Haga, S Kojima, Y Tateishi, M Shirakawa, T Haga

FEBS LETTERS 580(1) 23-26 2006年1月
Inhibitor-assisted refolding of protease: A protease inhibitor as an intramolecular chaperone

S Kojima, A Iwahara, H Yanai

FEBS LETTERS 579(20) 4430-4436 2005年8月
Stabilization of the fibrous structure of an α-helix-forming peptide by sequence reversal

小島修一, Shuichi Kojima, Yukino Kuriki, Kazumori Yazaki, Kin-ichiro Miura

Biochemical and Biophysical Research Communications 331(2) 577-582 2005年6月
Stabilization of the fibrous structure of an alpha-helix-forming peptide by sequence reversal

S Kojima, Y Kuriki, K Yazaki, K Miura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 331(2) 577-582 2005年6月
Mapping the position of the transition stare in the folding of a small α/β protein, POIA1

小島修一, Shuhei Tanaka, Shuichi Kojima, Atsuo Tamura

Chemical Physics 307(2-3) 233-242 2004年12月
Mapping the position of the transition state in the folding of a small alpha/beta protein, POIA1

S Tanaka, S Kojima, A Tamura

CHEMICAL PHYSICS 307(2-3) 233-242 2004年12月
Low pH facilitates uptake of proteins by cells through a non-endocytic pathway

M Motizuki, T Takei, K Tasaka, S Yokota, S Kojima, T Haga, K Tsurugi

JOURNAL OF BIOCHEMISTRY 135(6) 713-719 2004年6月
Cloning and expression of the enolase gene from Desulfovibrio vulgaris (Miyazaki F)

M Kitamura, Y Takayama, S Kojima, K Kohno, H Ogata, Y Higuchi, H Inoue

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1676(2) 172-181 2004年1月
Inhibitory specificity change of the ovomucoid third domain of the silver pheasant upon introduction of an engineered Cys(14)-Cys(39) bond

H Hemmi, T Kumazaki, T Yamazaki, S Kojima, T Yoshida, Y Kyogoku, M Katsu, F Shinohara, H Yokosawa, K Miura, Y Kobayashi

BIOCHEMISTRY 42(9) 2524-2534 2003年3月
Functional tolerance of Streptomyces subtilisin inhibitor toward conformational and stability changes caused by single-point mutations in the hydrophobic core

M Oda, A Tamura, K Kanaori, S Kojima, K Miura, K Momma, B Tonomura, K Akasaka

JOURNAL OF BIOCHEMISTRY 132(6) 991-995 2002年12月
Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-Ⅱ

小島修一, Mitsuyoshi Motizuki, Takanori Satoh, Toshiaki Takei, Takehito Itoh, Sadaki Yokota, Shuichi Kojima, Kin-ichiro Miura, Tatsuya Samejima, Kunio Tsurugi

The Journal of Biochemistry 132(1) 115-119 2002年7月
Structure-activity analysis of an antimicrobial peptide derived from bovine apolipoprotein A-II

M Motizuki, T Satoh, T Takei, T Itoh, S Yokota, S Kojima, K Miura, T Samejima, K Tsurugi

JOURNAL OF BIOCHEMISTRY 132(1) 115-119 2002年7月
Requirement for hydrophobic Phe residues in Pleurotus ostreatus proteinase A inhibitor 1 for stable inhibition

S Kojima, Y Hisano

PROTEIN ENGINEERING 15(4) 325-329 2002年4月
Structure of POIA1, a homologous protein to the propeptide of subtilisin: Implication for protein foldability and the function as an intramolecular chaperone

H Sasakawa, S Yoshinaga, S Kojima, A Tamura

JOURNAL OF MOLECULAR BIOLOGY 317(1) 159-167 2002年3月
Accelerated refolding of subtilisin BPN ' by tertiary-structure-forming mutants of its propeptide

S Kojima, H Yanai, K Miura

JOURNAL OF BIOCHEMISTRY 130(4) 471-474 2001年10月
Cloning and expression of the catalase gene from the anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F)

M Kitamura, T Nakanishi, S Kojima, Kumagai, I, H Inoue

JOURNAL OF BIOCHEMISTRY 129(3) 357-364 2001年3月
Alteration of inhibitory properties of Pleurotus ostreatus proteinase A inhibitor 1 by mutation of its C-terminal region

S Kojima, Y Hisano, K Miura

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 281(5) 1271-1276 2001年3月
Analysis of the isoform of Xenopus euakryotic translation initiation factor 4E

M Wakiyama, A Suzuki, M Saigoh, N Sakai, H Miyoshi, S Kojima, K Miura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 65(1) 232-235 2001年1月
Effects of amino acid replacements around the reactive site of chicken ovomucoid domain 3 on the inhibitory activity toward chymotrypsin and trypsin

S Kojima, N Takagi, T Minagawa, N Fushimi, K Miura

PROTEIN ENGINEERING 12(10) 857-862 1999年10月
Expression of a zinc-binding domain of boar spermatidal transition protein 2 in Escherichia coli

H Sato, K Akama, S Kojima, K Miura, A Sekine, M Nakano

PROTEIN EXPRESSION AND PURIFICATION 16(3) 454-462 1999年8月
Involvement of the C-terminal region of yeast proteinase B inhibitor 2 in its inhibitory action

S Kojima, M Deguchi, K Miura

JOURNAL OF MOLECULAR BIOLOGY 286(3) 775-785 1999年2月
High performance in refolding of Streptomyces griseus trypsin by the aid of a mutant of Streptomyces subtilisin inhibitor designed as trypsin inhibitor

D Nohara, H Sugiura, H Sakakibara, M Matsubara, S Kojima, K Miura, T Sakai

JOURNAL OF BIOCHEMISTRY 125(2) 343-347 1999年2月
P1-specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor

T Tanaka, H Matsuzawa, S Kojima, Kumagai, I, K Miura, T Ohta

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 62(10) 2035-2038 1998年10月
An endogenous target protease, SAM-P26, of Streptomyces protease inhibitor (SSI): Primary structure, enzymatic characterization, and its interaction with SSI

S Taguchi, S Yamada, S Kojima, H Momose

JOURNAL OF BIOCHEMISTRY 124(4) 804-810 1998年10月
Primary structure of Streptomyces griseus metalloendopeptidase II

S Kojima, T Kumazaki, S Ishii, K Miura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 62(7) 1392-1398 1998年7月
Cloning and expression of the gene encoding flavodoxin from Desulfovibrio vulgaris (Miyazaki F)

M Kitamura, T Sagara, M Taniguchi, M Ashida, K Ezoe, K Kohno, S Kojima, K Ozawa, H Akutsu, Kumagai, I, T Nakaya

JOURNAL OF BIOCHEMISTRY 123(5) 891-898 1998年5月
Tertiary structure formation in the propeptide of subtilisin BPN ' by successive amino acid replacements and its close relation to function

S Kojima, T Minagawa, K Miura

JOURNAL OF MOLECULAR BIOLOGY 277(5) 1007-1013 1998年4月
The propeptide of subtilisin BPN' as a temporary inhibitor and effect of an amino acid replacement on its inhibitory activity

S Kojima, T Minagawa, K Miura

FEBS LETTERS 411(1) 128-132 1997年7月
Disulfide bond formation is not involved in cap-binding activity of Xenopus translation initiation factor eIF-4E

Motoaki Wakiyama, Nobuya Sakai, Shuichi Kojima, Kin-Ichiro Miura

FEBS Letters 409(3) 407-410 1997年6月16日
Disulfide bond formation is not involved in cap-binding activity of Xenopus translation initiation factor eIF-4E

M Wakiyama, N Sakai, S Kojima, K Miura

FEBS LETTERS 409(3) 407-410 1997年6月
Molecular phylogenetic characterization of Streptomyces protease inhibitor family

S Taguchi, S Kojima, M Terabe, Y Kumazawa, H Kohriyama, M Suzuki, K Miura, H Momose

JOURNAL OF MOLECULAR EVOLUTION 44(5) 542-551 1997年5月
Cloning and expression of the rubredoxin gene from Desulfovibrio vulgaris (Miyazaki F) - Comparison of the primary structure of desulfoferrodoxin

M Kitamura, Y Koshino, Y Kamikawa, K Kohno, S Kojima, K Miura, T Sagara, H Akutsu, Kumagai, I, T Nakaya

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1351(1-2) 239-247 1997年3月
Fibril formation by an amphipathic α-helix-forming polypeptide produced by gene engineering

小島修一, Shuichi Kojima, Yukino Kuriki, Takao Yoshida, Kazumori Yazaki, Kin-ichiro Miura

Proceedings of the Japan Academy 73(1) 7-11 1997年1月
A novel member of the subtilisin-like protease family from Streptomyces albogriseolus

M Suzuki, S Taguchi, S Yamada, S Kojima, KI Miura, H Momose

JOURNAL OF BACTERIOLOGY 179(2) 430-438 1997年1月
Fibril formation by an amphipathic alpha-helix-forming polypeptide produced by gene engineering

S Kojima, Y Kuriki, T Yoshida, K Yazaki, K Miura

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES 73(1) 7-11 1997年1月
Synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity

S Kojima, K Miyoshi, K Miura

PROTEIN ENGINEERING 9(12) 1241-1246 1996年12月
Synthesis of α-helix-forming peptides by gene engineering methods and their characterization by circular dichroism spectra measurements

小島修一, Shuichi Kojima, Yukino Kuriki, Yoshihiro Sato, Fumio Arisaka, Izumi Kumagai, Sho Takahashi, Kin-ichiro Miura

Biochimica et Biophysica Acta 1294(2) 129-137 1996年5月
Synthesis of alpha-helix-forming peptides by gene engineering methods and their characterization by circular dichroism spectra measurements

S Kojima, Y Kuriki, Y Sato, F Arisaka, Kumagai, I, S Takahashi, K Miura

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1294(2) 129-137 1996年5月

書籍等出版物

廣川タンパク質化学第7巻制御タンパク質‐インヒビター 36ズブチリシンインヒビター‐（共著）

廣川書店 2002年
プロペチドおよびプロテアーゼによるプロテアーゼのフォールディング

蛋白質・核酸・酵素 2001年
プロテアーゼのプロペプチドの二面性:インヒビターおよび分子シャペロン

バイオサイエンスとインダストリー 1998年
シリーズ分子生物学6 構造生物学 4.6 プロテアーゼとインヒビター

朝倉書店 1998年
バイオテクノロジー教科書シリーズ9 蛋白質工学概論 (共著)

株式会社コロナ社 1995年

もっとみる

講演・口頭発表等

L-アミノ酸連結酵素YwfEの基質認識様式

第85回日本生化学会大会 2012年
L-アミノ酸連結酵素の結晶構造解析

第11回日本蛋白質科学会年会 2011年
抗生物質バチライシン生合成酵素の結晶構造解析

第84回日本生化学会大会 2011年
銅イオンポンプのドメイン間相互作用

第82回日本生化学会大会 2009年
分子内シャペロンとしてのPOIA1

第7回日本蛋白質科学会年会 2007年

もっとみる

所属学協会

共同研究・競争的資金等の研究課題

抗生物質の生合成に関わる酵素の構造と機能に関する研究

2009年
Relationship of the protease inhibitor and molecular chaperon activities in the propeptides

Grant-in-Aid for Scientific Research 1996年
プロペプチドおよびプロテアーゼインヒビターによる蛋白質の立体構造形成補助作用に関する研究

科学研究費補助金 1996年
Synthesis and Characterization of α-Helix-forming Peptide

Grant-in-Aid for Scientific Research 1989年
αヘリックス形成ペプチドによる集合体形成の分子機構に関する研究

科学研究費補助金 1989年

もっとみる

一覧へ戻る

小島 修一

基本情報

研究キーワード

研究分野

経歴

学歴

MISC

書籍等出版物

講演・口頭発表等

所属学協会

共同研究・競争的資金等の研究課題

小島修一