Fumitaka Mafune, Ken Miyajima, Kensuke Tono, Yoshihiro Takeda, Jun-ya Kohno, Naoya Miyauchi, Jun Kobayashi, Yasumasa Joti, Eriko Nango, So Iwata, Makina Yabashi
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY 72 520-523 2016年4月 査読有り
A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 mm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 angstrom from < 0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.