Faculty of Science

李 允求

Lee Yoonku

基本情報

所属
学習院大学 理学部 生命科学科 助教
学位
博士(農学)(東京大学)

研究者番号
50847168
J-GLOBAL ID
201901014787725941
researchmap会員ID
B000357795

論文

 5
  • Jung Lee, Toshiaki Fujimoto, Katsushi Yamaguchi, Shuji Shigenobu, Ken Sahara, Atsushi Toyoda, Toru Shimada
    Molecular Ecology 33(14) e17434 2024年7月  査読有り
  • Jung Lee, Tomoaki Nishiyama, Shuji Shigenobu, Katsushi Yamaguchi, Yutaka Suzuki, Toru Shimada, Susumu Katsuma, Takashi Kiuchi
    Molecular Ecology Resources 2020年9月28日  査読有り
    Samia ricini, a gigantic saturniid moth, has the potential to be a novel lepidopteran model species. Samia ricini is far more resistant to diseases than the current model species Bombyx mori, and therefore can be more easily reared. In addition, genetic resources available for S. ricini rival those for B. mori: at least 26 ecoraces of S. ricini are reported and S. ricini can hybridize with wild Samia species, which are distributed throughout Asian countries, and produce fertile progenies. Physiological traits such as food preference, integument colour and larval spot pattern differ among S. ricini strains and wild Samia species so that those traits can be targeted in forward genetic analyses. To facilitate genetic research in S. ricini, we determined its whole genome sequence. The assembled genome of S. ricini was 458 Mb with 155 scaffolds, and the scaffold N50 length of the assembly was ~ 21 Mb. In total, 16,702 protein coding genes were predicted. While the S. ricini genome was mostly collinear with that of B. mori with some rearrangements and few S. ricini-specific genes were discovered, chorion genes and fibroin genes seemed to have expanded in the S. ricini lineage. As the first step of genetic analyses, causal genes for "Blue," "Yellow," "Spot," and "Red cocoon" phenotypes were mapped to chromosomes.
  • 李允求
    13(10) e0205758-e0205758 2018年  査読有り
  • Hisayoshi Fukumori, Jung Lee, Tsuguru Fujii, Zenta Kajiura, Yutaka Banno
    Cryobiology 77 71-74 2017年8月  査読有り
  • J. Lee, T. Kiuchi, M. Kawamoto, T. Shimada, S. Katsuma
    INSECT MOLECULAR BIOLOGY 24(5) 561-569 2015年10月  査読有り
    We recently showed that the Masculinizer gene (Masc) plays a primary role in sex determination in the lepidopteran model insect Bombyx mori. However, it remains unknown whether this Masc protein-dependent sex determination system is conserved amongst lepidopteran insects or within the family Bombycidae. Here we cloned and characterized a Masc homologue (TvMasc) in Trilocha varians (Lepidoptera: Bombycidae), a species closely related to B. mori. To elucidate the role of TvMasc in the sex determination cascade of T. varians, TvMasc expression was knocked down in early embryos by the injection of small interfering RNAs (siRNAs) that targeted TvMasc mRNAs. Both female- and male-type splice variants of Tvdsx, a doublesex (dsx) homologue in T. varians were observed in control siRNA-injected embryos. By contrast, only female-type splice variants were observed in TvMasc siRNA-injected embryos. These results indicate that the TvMasc protein directly or indirectly regulates the splicing patterns of Tvdsx. Furthermore, we found that male-type splice variants of B. mori dsx (Bmdsx) were produced in TvMasc-overexpressing BmN4 cells. The mRNA level of B. mori Imp, a gene whose product induces male-specific Bmdsx splicing also increased. These results suggest that Masc genes play similar roles in the sex-determination cascade in Bombycidae.

所属学協会

 3

共同研究・競争的資金等の研究課題

 5