Extracellular lipase activity from Ralstonia sp. NT80 is induced significantly by fatty alcohols such as stearyl alcohol. We found that when lipase expression was induced by stearyl alcohol, a 14-kDa protein (designated EliA) was produced concomit...
The Journal of general and applied microbiology 59(2) 105-17 2013年 [査読有り]
We introduced single mutations into the rplC and rpsJ genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium Bacillus s...
Journal of bacteriology 194(22) 6282-91 2012年11月 [査読有り]
Among the 57 genes that encode ribosomal proteins in the genome of Bacillus subtilis, a Gram-positive bacterium, 50 genes were targeted by systematic inactivation. Individual deletion mutants of 16 ribosomal proteins (L1, L9, L15, L22, L23, L28, L...
Bioscience, biotechnology, and biochemistry 76(12) 2267-74 2012年 [査読有り]
The Streptomyces griseus 70S ribosome fraction was analyzed by radical-free and highly reducing two-dimensional (RFHR 2D) gel electrophoresis and mass spectrometry. Among the 60 putative ribosomal proteins that are encoded by the S. griseus genome...
An oligopeptide permease family ATP-binding cassette (ABC) transporter encoded by SGR2418-SGR2414 was shown to be essential for aerial mycelium formation on glucose-containing media in Streptomyces griseus. In spite of only weak sequence similarit...
Kato-Yamada Y   Akanuma G   Tagana T   Sawada M   Suzuki S   Shimada T   Tanaka K   Kawamura F   
Biochimica et Biophysica Acta - Bioenergetics 1859 e78 2018年8月
Ishizuka M   Akanuma G   Yoshizawa R   Nagakura M   Shiwa Y   Watanabe S   Yoshikawa H   Ushio K