JOURNAL OF MOLECULAR BIOLOGY 425(15) 2709-2721 2013年8月 [査読有り]
AMP phosphorylase (AMPpase) catalyzes the initial reaction in a novel AMP metabolic pathway recently found in archaea, converting AMP and phosphate into adenine and ribose 1,5-bisphosphate. Gel-filtration chromatography revealed that AMPpase from ...
A novel dioxygenase from Burkholderia ambifaria AMMD (SadA) stereoselectively catalyzes the C3-hydroxylation of Nsubstituted branched-chain or aromatic L-amino acids, especially N-succinyl-L-leucine, coupled with the conversion of alpha-ketoglutar...
IEEE Transactions on Applied Superconductivity 23(3) 3700104 2013年 [査読有り]
We have developed a protein crystal formation system, which can, on the ground, suppress convections, exerting magnetic forces on the solution including proteins. The protein formation system consists of a superconducting magnet system, protein cr...
Ferrous ion- and a-ketoglutarate-dependent dioxygenase from Burkholderia ambifaria AMMD (SadA) catalyzes the C3-hydroxylation of N-substituted branched-chain l-amino acids, especially N-succinyl-l-leucine, coupled to the conversion of alpha-ketogl...
PROTEIN EXPRESSION AND PURIFICATION 84(2) 214-218 2012年8月 [査読有り]
Flavin reductase HpaC(St) catalyzes the reduction of free flavins using NADH or NADPH. High hydrostatic pressure was used for the solubilization and refolding of HpaC(St), which was expressed as inclusion bodies in Escherichia coli to achieve high...
<p>Microgravity environment has been used to obtain high-quality crystals. Strong magnetic force produced by a superconducting magnet can cancel out the gravity force, enabling construction of quasi-microgravity environment on earth. We developed ...